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. 2015 Nov 16;10(11):e0143163. doi: 10.1371/journal.pone.0143163

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© 2015 Weiterer et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — A: Transversal section of untreated murine trachea displays complete and intact respiratory epithelium (arrow) as used for PTV experiments and for mRNA analysis from entire organs. B: Tracheal wall section after isolation of epithelium by brush removal shows the specificity of epithelial cell isolation for mRNA extraction. Thus, the arrow indicates a section of the trachea where the epithelium remained. All other tracheal wall structures were left intact. C & D: In isolated epithelium harvested from four different tracheae (1–4) mRNA of the adrenergic receptors β1(C), β2 (D) were found (α1D and α2A are not shown). epithelium: → scale bars: 25 μm. Table: Distribution of adrenergic receptors in the whole tracheae and in the isolated epithelium. +: mRNA expressed; -: mRNA not detectable.