Fig 5. GM or Zym reduce the Epi induced PTV increase.
30 minute pre-incubation with GM (A) or Zym (B) reduced in a dose dependent manner the response to Epi 10−8 M. The inhibiting effect of GM (C) (0.015 mg/ml, 0.02 mg/ml) or Zym (D) (0.1 mg/ml) onto the response to Epi was significant when compared to experiments when only Epi was applied (*p<0.05, **p<0.01). Very low concentrations of GM 0.01 mg/ml (p = 0.9444) or Zym 0.075 mg/ml (p = 0.6667) did not alter the Epi evoked increase in PTV. (E) (F) Neither high concentrations of GM (0.02 mg/ml) nor of Zym (0.1 mg/ml) affected the PTV nor the response of ATP as an index of viability of individually investigated tracheae. Exposure periods are presented by horizontal bars. Mann Whitney U-test. Mean ± S.E.M.. (control: untreated tracheae (n = 7); all other experiments (n = 5), n: tracheae from n animals).