Table 1. Accession numbers, and primer sequences of target genes (TG) and reference genes (RG) used in real time PCR.
Optimal annealing temperatures (TA) were predetermined by gradient PCR, and a TA of 55°C was used for all genes.
| Gene Name | Genbank accession number | Forward primer (5’-3’) | Reverse primer (5’-3’) |
|---|---|---|---|
| Endochitinase (TG) | FG512537 | GGTTGCTGCTTTTCTTGCTC | CACAAGGCCACTGTTGATTG |
| β-1,3-glucosidase (TG) | FG455092 | TTGGTTCAACATGTCAAAGGAG | TAGGCTGCTTGTTGGGAAAG |
| Acyl lipid metabolism (TG) | FG475093 | CAGGATTTAGTAGCAATGATGGAC | AAGGGATCCTCTCGTAATCCA |
| TIR-NBS-LRR resistance protein (TG) | FG525643 | GTCTGGCAGGGTTGGTCTTG | GTAACACTGAGGACCGTGCG |
| PAD4 triacylglycerol lipase (TG) | FG479414 | ATGCTCGTGACAGGAAACGC | GCAATGCCAATGTAACACCTGC |
| CC-NBS-LRR resistance protein (TG) | FG407814 | AGACTTTGAAGATGCCCCCTTGC | TGTAGCCTGCCAATTGACTTTGG |
| Actin (RG) | FG520231 | TGCATGAGCGATCAAGTTTCAAG | TGTCCCATGTCTGGTTGATGACT |
| Elongation factor EC 3.6.5.3 (RG) | FG526520 | ACAAGCTGGTGACAATGTGG | CGACCACCTTCATCCTTTGT |