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. 2015 Nov 16;10(11):e0142991. doi: 10.1371/journal.pone.0142991

Fig 5. Cell type-dependent differences in BAF complexes.

Fig 5

A and B) The mRNA (A) and protein (B) expression of BAF47 in KhES1-HA, KhES1-NCC-FL, and KhES1-MSC-FL cells. Cells with Stuffer (-Control) and SS18-SSX2 cells were treated with the indicated concentrations of DOX for 24 h, and the expression of BAF47 was analyzed. A) mRNA expression analysis using RT-qPCR. Expression levels were normalized to those of human ACTB. Error bars reflect SD in 3 experiments. B) Protein expression analysis by Western blotting. The BAF47 protein was detected using an anti-BAF47 antibody. 293T cells were used as a positive control. C) Recruitment of SS18-SSX2 to the FZD10 core promoter region (from -93 to +47 bp) in KhES1-HA, KhES1-NCC-HA, and KhES1-MSC-HA cells. Cells were treated with DOX (0.1, 1.0, and 3.0 μg/ml for KhES1-HA, KhES1-NCC-HA, and KhES1-MSC-HA cells, respectively) for 24 h. A ChIP assay with an anti-HA antibody and RT-qPCR were performed. The values indicate relative to rabbit IgG. Error bars reflect SD in 3 experiments.