PASA of rat femur bone specimens. (A) Examples of power spectral density (PSD) of the RF PA signal of three groups (OVX, Sham, OVX + ZOL) after calibration by removing the system response. The corresponding linear fit (R2 = 0.24, 0.78, 0.68, respectively, for OVX, Sham, and OVX + ZOL) in the spectral range of 2.8-31.5 MHz leads to quantified spectral parameter slope. (B) The quantified spectral parameter slope of the three groups of bones.