AO corrects wavefront aberration with the closed-loop frequency of 100 Hz and 20 Hz. (a) The subject’s eye was with a nature pupil and accommodation. (b) The pupil of the same subject was dilated with 1.0% tropicamide and 2.5% phenylephrine hydrochloride. The accommodation was paralyzed. The subject fixated on the upper-left corner of the light scanning rater, i.e., the infinity. For the 20 Hz AO, the wavefront aberration was measured at 100 Hz but the correction was conducted at 20 Hz. DM1 (Hi-Speed DM97-15, ALPAO SAS, France) was used to correct the wave aberration during the image acquisition.