Fig 4. Virulence of C. albicans mutant strains in the infection model.
(A) Survival rates of embryos. Embryos alone (Control) or embryos with 5 × 105 cells/mL of bcr1/bcr1, cph1/cph1 efg1/efg1, efg1/efg1, tec1/tec1, cph1/cph1, sap6/sap6, or WT (SC5314) cells in RPMI/serum were incubated at 30°C for 4 h. Survival rates were determined after an additional 1 day and 2 days of incubation. (B) Representative embryos were co-incubated with (a) bcr1/bcr1, (b) cph1/cph1 efg1/efg1, (c) efg1/efg1, (d) tec1/tec1, (e) cph1/cph1, (f) sap6/sap6, or (g) WT (SC5314) cells, and photographed after an additional 2 days of incubation. Scale bar = 200 μm. The data are from 4 repeat experiments. Approximately 70 embryos were tested for each strain.