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. 2015 Nov 16;10(11):e0142400. doi: 10.1371/journal.pone.0142400

Fig 4. Activation of human D2 promoter by GATA2 or GATA3 endogenously expressed in choriocarcinoma-derived JEG3 cells.

Fig 4

(A) A schematic representation of GATA-REs and their mutants (RM1, RM2 and RM3). (B) Using the calcium phosphate method, 2.0 μg hD2-hRluc and its mutants (M1, M2 and M3) were transfected into JEG3 cells. *, P<0.05 for hD2-hRluc (Wt) vs. mutants. The results are means ± S.E. for three independent experiments. pGL4.74[hRLuc/TK] (2.0 μg/well) was used as the inter-assay control and its expression level was adjusted to a value of 100.