Figure 5.

The inhibition of LY294002 on the regulation of O-GlcNAcylation on invasion of thyroid anaplastic cancer cells.

Notes: For 24 hours, 5 μM Thiamet-G (Thia-G) treated or untreated and OGT overexpression or underexpressed 8305C cells were treated with 2 μM LY294002. (A) The inhibition of LY294002, a specific inhibitor of PI3K, on Akt1 phosphorylation (Ser473) was assessed by Western blot. (B) Transwell assay analyzed the impact of LY294002 on invasion of Thiamet-G treated (on the left) or OGT overexpression (on the right) in 8305C cells. Western blot analysis showed the corresponding phosphorylation (Ser473) of Akt1, demonstrating the changes of Akt1 activity in respectively treated cells with Thiamet-G, OGT overexpression or LY294002. Anti-p-Akt1 (Ser473), anti-Akt1, and anti-GAPDH antibodies were used in the experiments. Error bars represent the mean ± standard error of the mean of three independent experiments, *P<0.05, **P<0.01.

Abbreviations: Ctrl, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.