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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Mol Microbiol. 2015 Jun 6;97(4):660–675. doi: 10.1111/mmi.13053

Figure 1. Growth phase dependent polar localization of chemotaxis cluster III proteins.

Figure 1

(A) Schematic of chemotaxis cluster III. (B) Localization of ectopically expressed YFP-CheW1, CFP-CheW3, and YFP-CheA3 in cells from exponential (left) and stationary (right) phase cultures of wild type V. cholerae. (C, D) Localization of CFP-CheW3 in cluster III-deficient strain SR29 (C) and in SR36, a strain deleted for chemotaxis clusters I and II (D). Cells were grown to stationary phase. (E) Localization of YFP-CheW1 and CFP-CheW3 expressed from their respective native loci (in strain SR15) in stationary phase cells. Yellow arrowheads indicate cells with YFP-CheW1 and CFP-CheW3 localized to the same pole. White arrowheads indicate cells where YFP-CheW1 and CFP-CheW3 localize to opposite poles.