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. Author manuscript; available in PMC: 2015 Nov 16.
Published in final edited form as: Biochim Biophys Acta. 1982 Dec 8;693(1):93–98. doi: 10.1016/0005-2736(82)90474-6

Fig. 1.

Fig. 1

Quick-frozen vesicles. X60000. Bar = 0.2 μm. A. A 0.2 μm vesicle, quick-frozen. The diameter of the vesicles ranges from 0.08 to 0.2 μm. B. Several PC:CL vesicles fusing after being injected with 5 mM CaC1 2 (final concentration) 1 s prior to quick-freezing. The average diameter of fused vesicles was 0.4 μm. C. Two large egg PE:PS vesicles captured by quick-freezing in the process of fusion . D. Lipidic particles decorate this PC:CL vesicle incubated for 2 h in Ca2+ and glycerol. E. The particles apparent following a 2 h incubation in Ca2+/glycerol outline a cleared circle the same size (0.2 μm) as unfused vesicles. F. A few clustered particles adorn some PC:CL vesicles after a 2 h incubation in Ca2+ alone. G. The sequence of fusion events in the outer leaflet of PC:CL vesicles pre-incubated with glycerol for 2 h and then injected with Cai2+ 1 s prior to0 quick-freezing. Two vesicles on the bottom right have aggregated. At the top left, three fused vesicles remain separated by narrow necks, which widen in the adjacent vesicle. H. PC:CL vesicles incubated for 2 h in glycerol alone and then injected with Ca2+ 1 s before quick-freezing, fuse, displaying a few particles in the region of confluence. These particles embellish both P (arrow) and E fracture faces.