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Relative mean concentration values of nearly 60 abundant blood metabolites quantitated using NMR after protein precipitation using methanol (MeOH) or acetonitrile (ACN) at different solvent to serum ratios as indicated. The values are relative to the mean = 1 for MeOH at a 2:1 ratio. Note methanol performs most optimally over a wide range and the methanol to serum ratio of 2:1 provides the best performance [modified from Ref. [23]].
