Fig 3. Demethylation of PP2A induced by alkali treatment.

Microcystin beads incubated with sperm extracts from proximal caput, distal caput and caudal regions of epididymis were subjected to NaOH treatment (+). Equal amounts incubated without NaOH are untreated controls (-). Details of the procedure are described in Materials and Methods. Different volumes were loaded in Western blot for each epididymal region, hence shown as separate panels. Duplicate blots were processed, one was probed with anti-demethyl PP2A antibody and the other was probed with anti-PP2A antibody.