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. 2015 Nov 16;10(11):e0141961. doi: 10.1371/journal.pone.0141961

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© 2015 Dudiki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 4 — A) Western blot showing detectable levels of LCMT1 in mouse whole sperm extracts prepared in 1%SDS and testis extracts. Mouse brain extract was loaded as positive control for Anti-LCMT1 antibody. B) Presence of immunoreactive PME1 at ~43kd in HB+ sonicated soluble bull sperm extracts from all three regions of epididymis is shown with Anti-PME1 antibody. The blot was stripped and probed with Anti-PP2A antibody to show equal protein loading. (C) Sperm isolated from the three regions of the epididymis were incubated with DMSO (control) or 500 nM ABL127 for 1hr. Following this incubation, sperm extracts were prepared and analyzed by Western blot with anti-demethyl PP2A antibody. The first panel shows 10⁶ proximal caput sperm/lane at 10 seconds exposure. The remaining panels show 2X10⁶ distal caput or caudal sperm/lane at 60 seconds exposure. A duplicate blot probed with Anti-PP2A is used as loading control.