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. 2015 Nov 16;10(11):e0141961. doi: 10.1371/journal.pone.0141961

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© 2015 Dudiki et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 10 — Sperm isolated from proximal caput, distal caput and cauda were treated with either 5nM OA to specifically inhibit PP2A or 1uM OA to inhibit both PP1 and PP2A. The extracts were analyzed by western blot analysis with anti-phospho GSK3α/β (pSer 21/9) antibody. 10⁶ proximal caput sperm/lane and 4X10⁶ distal caput or caudal sperm/lane was loaded.