Skip to main content
. Author manuscript; available in PMC: 2016 Mar 10.
Published in final edited form as: N Engl J Med. 2015 Sep 10;373(11):1040–1047. doi: 10.1056/NEJMoa1504542

Figure 3. CD19 Expression on Neoplastic Plasma Cells.

Figure 3

Panel A shows surface CD19 expression (right) on multiple myeloma plasma cells (CD38+CD45− gate, left) obtained from the pre-ASCT bone marrow aspirate. Brackets (right) indicate fluorescence thresholds that were used for fluorescence-activated cell sorting (FACS) of multiple myeloma plasma cells according to surface CD19 expression for analysis of CD19 messenger RNA levels. Panel B shows immunoglobulin light-chain expression in the overall population of malignant plasma cells (left) and the CD19+ subset of malignant plasma cells (right) from the pre-ASCT bone marrow aspirate. Panel C shows the results of reverse-transcriptase–polymerase-chain-reaction analysis of CD19 expression in FACS subsets of peripheral-blood mononuclear cells (PBMCs) from healthy donors and malignant plasma cells from the pre-ASCT bone marrow aspirate; results are normalized to expression in the CD19+ healthy-donor PBMC sample and plotted on a semi-logarithmic scale. ND denotes not detectable. I bars indicate 95% confidence intervals.