Figure 5.

Blocking LTB₄ synthesis and inhibiting p38 MAPK signaling decreases fibroblast activation and Nox4 in experimental PAH. A, LTB₄ concentrations in the bronchoalveolar lavage fluid (BALF) of DMSO, SU or SU+SB203580 animals. B, Immunofluorescence images of rat lung tissues stained with 5-LO (magenta), Vimentin (green) and Nox4 (red). Yellow dash lines approximate the adventitial zone. C, Representative immunofluorescence images of lung sections stained with Nox4 (green). Amplex Red (red) indicates tissue H₂O₂ level. DAPI (blue) stains nuclei; DIC highlights alveolar and vascular structures; n= 5. Data are expressed as means ± SEM. (*: p<0.05)