Table 2.
Recombinant VEGFA Protein | Effect on Cord Formation | Effects on Vasculature | PECAM Staining |
---|---|---|---|
VEGFA120 | Swollen cords with some perturbed (R&D Systems, #494-VE) | Thickened coelomic vessel, more vasculature between cords | 157% |
VEGFA164 | Some cords misshapen or perturbed (R&D Systems, #564-RV) | Thickened coelomic vessel, more vasculature between cords that formed | 160% |
VEGFAxxxb antibody (5 ng/ml) | Swollen or perturbed cords, misshapen cords (Abcam, #ab14994) | Increased vasculature all throughout testis | 195% |
VEGFAxxxb antibody (50 ng/ml) | Swollen or perturbed cords, misshapen cords (Abcam, #ab14994) | Increased vasculature all throughout testis | 195% |
VEGFA = vascular endothelial growth factor A