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. 2015 Jul 17;1(6):e1500165. doi: 10.1126/sciadv.1500165

Fig. 3. Correlative light and electron microscopic images showing the distribution of QD-labeled HER2 on an SKBR3 cell.

Fig. 3

(A) Selected area of the fluorescence image in Fig. 2B. This region contained membrane ruffles as shown in the DIC image of Fig. 2C. (B) ESEM-STEM image at the same area, recorded at ×15,000 magnification. (C) Pair correlation function g(x) (line) determined from the 122 detected label positions in the rectangular region in (B). The dashed line at unity serves as a guide to the eye for a random distribution. (D) STEM image recorded of the boxed region shown in (B) at ×75,000 magnification. The localization of individual HER2 receptors became visible as the bright, bullet-shaped QDs. (E) Automatically detected labels were outlined in light green. Numerous pairs of HER2 were observed (two examples are indicated by arrowheads). Scale bars, 2 μm (A and B); 200 nm (D and E).