Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Oct 2;1(9):e1500415. doi: 10.1126/sciadv.1500415

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2015, The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

PMC Copyright notice

Fig. 1 — (A) Mono-7D6-Fab binds to T cells. Thy1.2⁺CD4⁺ or Thy1.2⁺CD8⁺ B6 cells were analyzed for binding irrelevant Ms IgG, Mono-7D6-Fab, or 7D6 mAb detected by anti–Ms IgG fluorescein isothiocyanate. (B and C) Mono-7D6-Fab does not block antigen binding to transgenic TCRs. (B) Pmel-1 TCR transgenic cells were preincubated with control Ms IgG Fab or Mono-7D6-Fab, and Thy1.1⁺CD8⁺ cells were analyzed for binding H-2K^D/hgp100 tetramer. (C) OT-I TCR transgenic cells were preincubated with control Ms IgG Fab or Mono-7D6-Fab, and Thy1.2⁺CD8⁺ cells were analyzed for binding H-2K^B-OVA tetramer. (D and E) Mono-7D6-Fab does not alter T cell response to stimulation of transgenic TCRs by strong pMHC ligands. (D) Pmel-1 cells were cultured with no peptide or hgp100 peptide (500 ng/ml) in the presence of control Ms IgG or Mono-7D6-Fab and analyzed at 24 hours for Pmel-1 Vβ13 TCR down-regulation. (E) OT-I T cells were cultured with no peptide or 0.2 nM pOVA in the presence of control Ms IgG or Mono-7D6-Fab and analyzed at 24 hours for OT-I Vα2 TCR down-regulation. (F) Mono-7D6-Fab binding does not stimulate T cells. B6 cells were incubated with Ms IgG Fab, Mono-7D6-Fab, or 2C11 mAb and analyzed for induction of CD69 or CD25 expression. From (A) to (F), one representative experiment out of three or more performed experiments is shown.