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. 2015 Oct 2;1(9):e1500415. doi: 10.1126/sciadv.1500415

Fig. 4. Mono-7D6-Fab injection is inert in unimmunized mice in vivo.

Fig. 4

(A) Three B6 mice per experimental group were injected retro-orbitally with 10 μg of control Ms IgG Fab, Mono-7D6-Fab, 7D6 mAb, or 2C11 mAb. After 24 hours, blood samples were collected, and cells were counted in triplicate and analyzed by flow cytometry. The T cell/B cell ratio found in the blood is displayed (mean ± SEM). (B and C) Three B6 mice per experimental group were injected retro-orbitally with 10 μg of control Ms IgG Fab or Mono-7D6-Fab on days 0, 2, 4 and 6, and were sacrificed on day 7. (B) Serum cytokines found in plasma after 7 days. For comparison, serum cytokines from mice sacrificed 3 hours after a single injection of PBS or 50 μg of staphylococcal enterotoxin B (SEB) were quantified in parallel (mean ± SEM). (C) Liver, kidney, and lung tissues were collected in buffered formalin, paraffin-embedded, cut, and stained with hematoxylin and eosin (H&E) following standard procedures for histopathologic analysis. H&E-stained sections were evaluated by light microscopy. Scale bar, 100 μm. G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; KC, keratinocyte chemoattractant; MCP-1, monocyte chemoattractant protein-1; MIP-1a, macrophage inflammatory protein-1α; MIP-1b, macrophage inflammatory protein-1β; TNF-α, tumor necrosis factor–α.