Fig. 5. Mono-7D6-Fab reduces tumor burden in a mouse model of melanoma lung metastasis.

(A and C) B6 mice were intravenously injected with B16-F10 melanoma cells and then with control Ms IgG Fab or Mono-7D6-Fab. After 21 days, the lungs were photographed and weighed. (A) Lung photographs from one independent experiment. (B and C) Dots represent individual mice with data pooled from three independent experiments (mean ± SEM). (B) Lung weight. (C) Quantification of melanoma found in lungs (B and C; ***P < 0.0005, two-tailed unpaired Student’s t test). (D) Percentage of CD44⁺ and (E) percentage of PD1⁺CD11a⁺ T cells found in pooled mediastinal lymph nodes from mice engaged in one of three representative experiments, as shown in (A) to (C) (mean ± SD from triplicate samples; **P < 0.005, ***P < 0.0005, two-tailed unpaired Student’s t test).