Figure 2.

Chronic Depolarization Also Induces Rapid AIS Plasticity

(A) Maximum intensity projections (left) of DGCs labeled for Prox1 and AnkG after 3-hr treatment with +15 mM NaCl or KCl. Lines, axon start; arrowheads, AIS start and end positions; scale bar, 10 μm. Plot (right) shows cumulative fraction and (inset) mean + SEM of AIS lengths. Numbers in bars, n; Mann-Whitney test; ^∗∗∗p < 0.001.

(B) Maximum intensity projections (left) of Prox1-CreER^T2 DGCs infected with AAV-Flex-eGFP, labeled for Prox1 and AnkG after 3-hr treatment with +15 mM NaCl or KCl. All conventions as in (A).

See also Figures S2 and S3.