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The effect of FW (pH 7.0) and AFW (pH 4.0) on mRNA expression of the indicated genes in 3d post-fertilization (dpf) zebrafish embryos. The expression of atp6v1a, ae1b, nhe3b, ca2-like a, ca15a, cyp11b, gr, and mr mRNA was analyzed by qPCR, and the values were normalized to β-actin. Values are the mean ± SD (n = 6). Student's t-test, *p < 0.05; **p < 0.01; ***p < 0.001.
