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Effects of exogenous cortisol on (A) the mRNA expression of transporter genes, (B) proton secretion in the embryonic skin, and (C) proton secretion at single H+-ATPase-rich cells (HRCs) in 3-dpf zebrafish embryos. The expression of mRNA was analyzed by qPCR using β-actin as the internal control. SIET was used to measure the H+ activity at the skin and at single cells. Values are the mean ± SD (n = 6–12). Student's t-test, *p < 0.05; **p < 0.01; ***p < 0.001.
