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. 2015 Nov 17;6:328. doi: 10.3389/fphys.2015.00328

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Copyright © 2015 Lin, Shih, Liu, Hsu and Hwang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) morpholino oligonucleotides (MO) on (A) mRNA expression of transporters, (B) proton secretion in the embryonic skin, and (C) proton secretion at single H⁺-ATPase-rich cells (HRCs) in 3-dpf zebrafish embryos. The expression of mRNA was analyzed by qPCR using β-actin as the internal control. SIET was used to measure H⁺ activity at the skin and at single cells. Different letters indicate a significant difference (p < 0.05), as determined using One-way ANOVA followed by Tukey's multiple-comparison test. Values are the mean ± SD (n = 6–12).