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Effects of MR and GR MOs on (A) mRNA expression of transporters and (B) proton secretion in the embryonic skin in 3-dpf zebrafish embryos treated with FW (pH 7.0) or AFW (pH 4.0). Expression of mRNA was analyzed by qPCR using β-actin as the internal control. SIET was used to measure H+ activity at the skin. Different letters indicate a significant difference (p < 0.05), as determined using One-way ANOVA followed by Tukey's multiple-comparison test. Values are the mean ± SD (n = 6–12).
