FIGURE 3.
Visualization of the experimental set-up of a twin-chamber with tomato plants used by Dababat and Sikora (2007) and Vos et al. (2012b) to study the differential attraction of Meloidogyne incognita to mycorrhizal roots. The two plant compartments are connected by a bridge. The experiment consisted of three treatments: (i) control plants in both compartments (control treatment), (ii) mycorrhizal plants in both compartments (AMF treatment) and (iii) a control plant in the left compartment and a mycorrhizal plant in the right compartment (mixed treatment). Two days after transplanting, the compartments and bridge were watered to field capacity at time of inoculation and 2,000 freshly hatched M. incognita 2nd-stage juveniles were inoculated per twin chamber, exactly in the middle of the bridge. Twelve days after inoculation, nematode penetration was assessed in control and mycorrhizal root systems of all treatments.