Figure 7.

PKA signaling regulates reverse, but not forward electron flow, through complex I in mouse PmFBs. Mitochondrial H₂O₂ emitting potential (mOEP) was measured in RG (A) and WG (B) with succinate (left) or glutamate/malate/rotenone (right) and in the absence (white bars) or presence (gray bars) of 10 μM H89 in the assay media. Auranofin (1 μM) was added to specifically measure H₂O₂ production, and an “oxidant scavenging index” was determined based on the increase in mOEP following addition of auranofin. N = 4–8/condition. * denotes p < 0.05 compared to Control.