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Luciferase reporter assay in HepG2 cells. Cells were cotransfection of HepG2 cells with miR-20b mimics and wild-type HIF-1α, VEGF 3′-UTR led to a marked decrease in luciferase activity and cotransfection with miR-20b and mutant HIF-1α, VEGF 3′-UTR had no effect on luciferase activity, and cotransfection with NC miRNA and wild-type HIF-1α, VEGF 3′-UTR or mutant HIF-1α, VEGF 3′-UTR also showed no difference, ∗∗ P < 0.05 compared with negative control.
