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. 2015 Dec;28(12):1784–1793. doi: 10.5713/ajas.15.0286

Figure 1.

Figure 1

Preliminary study to determine the dose of lipopolysaccharide (LPS) used in inducing broilers immune stress. Twenty four 22-day-old male Cobb broilers were randomly assigned to four groups (control, 0.25 mg/kg body weight [BW] LPS, 0.5 mg/kg BW LPS, and 1 mg/kg BW LPS). At the age of 22, broilers in the 0.25 mg/kg BW LPS, 0.5 mg/kg BW LPS, and 1 mg/kg BW LPS groups were intraperitoneally injected with LPS at the dose of 0.25, 0.5, and 1 mg/kg BW, respectively, whereas those in the control groups were intraperitoneally injected with saline at the dose of 0.25 mg/kg BW. Blood samples were taken from a wing vein puncture at 24 h post LPS or saline injection and serum interleukin-1β (IL-1β) levels were determined. The feed intake was recorded from the ages of 22 to 28. Data were expressed as mean values with standard error of mean (n = 6). a,b,c Means with different superscripts in IL-1β levels differ significantly (p<0.05). A,B Means with different superscripts in average daily feed intake (ADFI) differ significantly (p<0.05).