Morphology of 1×, 2×, and 3× blastocysts. Morphology of 1× (A: right; B), 2× (A: left; C), and 3× (A: above; D) blastocysts. In vitro-developed blastocysts from each group were collected on day 7. This photograph was taken with a digital camera at the same scale. Scale bars = 100 μm. Abbreviations: 1×, zona-free, non-aggregated; 2×, two-embryo aggregate; 3×, three-embryo aggregate.