Table 1.

Reverse transcription-polymerase chain reaction conditions and primers

Genes	Primer sequences	Annealing temperature (°C)	Products size (bp)
Oct4	5′-AACGATCAAGCAGTGACTATTCG-3′ 5′-GAGTACAGGGTGGTGAAGTGAGG-3′	60	153
Nanog	5′-AATCTTCACCAATGCCTGAG-3′ 5′-GGCTGTCCTGAATAAGCAGA-3′	60	141
Sox2	5′-CGGCGGCAGGATCGGC-3′ 5′-GAGCTCCGCGAGGAAAA-3′	60	113
β-Actin	5′-GTGGACATCAGGAAGGACCTCTA-3′ 5′-ATGATCTTGATCTTCATGGTGCT-3′	60	137