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. 2015 Nov;28(11):1641–1648. doi: 10.5713/ajas.15.0181

Table 1.

Dietary composition and nutrient levels of the experimental diets (as-fed basis)

Item Treatment group
SBM SBM-CSP CSP SBM-DRM DRM CSP-DRM
Ingredient (%)
 Corn 62.64 65.00 67.00 58.00 56.60 63.10
 SBM, 44.82% CP 25.00 11.76 - 12.84 - -
 CSP, 52.73% CP - 10.00 18.70 - - 9.92
 DRM, 38.73% CP - - - 14.87 29.20 13.52
 Soybean oil 0.02 0.98 0.67 1.35 1.82 0.90
 L-Lysine-HCl, 78% 0.041 0.300 0.533 0.141 0.265 0.409
 DL-Methionine, 98% 0.167 0.186 0.203 0.146 0.126 0.166
 L-Tryptophan, 99% 0.006 0.024 0.042 0.013 0.025 0.034
 L-Threonine, 98% 0.032 0.129 0.217 0.050 0.078 0.152
 L-Isoleucine, 99% 0.025 0.156 0.275 0.089 0.163 0.221
 L-Valine, 99% 0.011 0.099 0.182 0.040 0.080 0.133
 L-Cysteine, 99% 0.149 0.161 0.174 0.100 0.059 0.120
 Arginine, 99% - - - - 0.082 -
 Calcium hydrogen phosphate 1.00 1.00 1.00 1.00 1.00 1.00
 Calcium carbonate 9.07 9.09 9.10 8.90 8.75 8.94
 Salt 0.30 0.30 0.30 0.30 0.30 0.30
 Zeolite powder 0.969 0.245 1.034 1.591 0.882 0.515
 Premix2 0.57 0.57 0.57 0.57 0.57 0.57
 Total 100 100 100 100 100 100
Calculated analysis3
 ME (kcal/kg) 2655 2655 2655 2655 2655 2655
 CP (%) 16.5 (16.52) 16.5 (16.53) 16.5 (16.47) 16.5 (16.53) 16.5 (16.48) 16.5 (16.49)
 EE (%) (1.07) (2.02) (1.47) (2.25) (3.54) (1.86)
 Calcium (%) 3.48 (3.45) 3.47 (3.46) 3.46 (3.47) 3.48 (3.45) 3.48 (3.49) 3.47 (3.45)
 Non-phytate phosphorus (%) 0.41 0.39 0.38 0.39 0.38 0.39
 Lysine (%) 0.896 (1.03) 0.930 (1.05) 0.959 (1.16) 0.922 (1.06) 0.944 (1.16) 0.952 (1.34)
 Methionine (%) 0.427 (0.45) 0.440 (0.45) 0.450 (0.40) 0.439 (0.39) 0.449 (0.39) 0.449 (0.42)
 Methionine + cysteine (%) 0.807 (0.78) 0.825 (0.78) 0.839 (0.80) 0.834 (0.77) 0.860 (0.82) 0.849 (0.84)
 Isoleucine (%) 0.717 (0.71) 0.703 (0.73) 0.744 (0.67) 0.735 (0.74) 0.751 (0.79) 0.746 (0.68)
 Threonine (%) 0.666 (0.73) 0.679 (0.68) 0.689 (0.71) 0.697 (0.70) 0.726 (0.78) 0.706 (0.74)
 Tryptophan (%) 0.194 (0.199) 0.198 (0.196) 0.202 (0.200) 0.201 (0.200) 0.284 (0.258) 0.204 (0.200)
 Valine (%) 0.802 (0.85) 0.823 (0.89) 0.843 (0.85) 0.827 (0.82) 0.850 (0.85) 0.845 (0.84)
 Arginine (%) 1.090 (1.07) 1.254 (1.24) 1.388 (1.39) 0.988 (0.98) 0.945 (1.07) 1.141 (1.32)
 Histidine (%) 0.473 (0.49) 0.445 (0.47) 0.418 (0.40) 0.455 (0.39) 0.430 (0.41) 0.424 (0.35)
 Leucine (%) 1.473 (1.45) 1.325 (1.36) 1.188 (1.01) 1.404 (1.45) 1.328 (1.44) 1.258 (1.18)
 Cystine (%) 0.380 (0.33) 0.385 (0.33) 0.389 (0.40) 0.395 (0.38) 0.411 (0.43) 0.400 (0.42)
 Phenylalanine (%) 0.793 (0.82) 0.781 (0.93) 0.764 (0.56) 0.723 (0.83) 0.639 (0.83) 0.706 (0.71)
Anti-nutritional factor
 Free gossypol (mg/kg) - 30.25 56.57 - - 30.01
 Isothiocyanate (mg/kg) - - - ND ND ND
 Oxazolidine thioketone(mg/g) - - - 0.050 0.099 0.046

HCl, hydrochloride; ME, metabolizable energy; CP, crude protein; EE, ether extract; ND, not detected;

1

The dietary types included soybean meal (SBM), cottonseed protein (CSP), double-zero rapeseed meal (DRM) individually or in combination with equal crude protein (SBM-CSP, SBM-DRM, and CSP-DRM) as the protein ingredient(s).

2

Provided per kilogram of diet: vitamin A, 12,500 IU; vitamin D3, 4,125 IU; vitamin E, 15 IU; vitamin K, 2 mg; vitamin B1, 0.98 mg; vitamin B2, 8.5 mg; calcium pantothenate, 50 mg; niacin 32.5 mg; pyridoxine, 8 mg; biotin, 2 mg; folic acid 5 mg; vitamin B12, 5 mg; copper, 8 mg; iodine, 1 mg; iron, 60 mg; selenium, 0.3 mg; manganese, 65 mg; zinc, 66 mg; choline, 0.5 g; phytase, 0.5 g; yeast culture®, 2.0 g.

3

Data regarding nutrients represent both the calculated value and analyzed value (in parentheses) except for ME (metabolizable energy) and non-phytate P.