Table 2.
Primers of genes chosen for quantitative real-time polymerase chain reaction
| GenBank accession number | Gene ID | Sequence (5′ to 3′) | Amplicon length (bp) | Tm (°C) |
|---|---|---|---|---|
| NM_205518 | ACTB |
F: CAAAGCCAACAGAGAGAAG R: CATCACCAGAGTCCATCA |
137 | 83.5 |
| NM_204305 | GAPDH |
F: ACTTTGGCATTGTGGAGGGT R: GGACGCTGGGATGATGTTCT |
131 | 86.0 |
| NM_204848 | HPRT |
F: CTCACAGGCTTCAACTCT R: AATGACCAAGACGAGATTCT |
115 | 80.0 |
| FJ977570.1 | IGF1 |
F: TCTCAACATCTCACATCTCT R: AAGCAGCACTTAACTAATTGT |
136 | 78.0 |
| NM_001199909 | PDK4 |
F: TGCAATCACCAAAGGTCACCA R: TGCAGTAGCTGAAGCTGTGTT |
87 | 79.0 |
| NM_204728 | PPARD |
F: TCTCCAAGCACATCTACAA R: CGGTCAAGATACCTCTCG |
75 | 78.5 |
| NM_00166326 | PPIA |
F: GGCTCCCAGTTCTTCATC R: ATCTGCTTGCTCGTCTTG |
143 | 88.5 |
| NM_205103 | TBP |
F: TGAATAGCACACGCACTA R: GAGGCAACACATACTGAAC |
178 | 81.5 |
F, forward; R, reverse; Tm, melting temperature.