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. 2015 Nov 1;128(21):3888–3897. doi: 10.1242/jcs.162586

Fig. 2.

Fig. 2.

Cx36-HaloTag fusion protein forms functional gap junctions that are normally regulated by PKA activities. (A) Live cell labeling of HeLa cells transfected with Cx36-Halo with HaloTag TMR ligand (red) followed by fixation and labeling with Cx36 antibody (green). The labels are co-localized at cell–cell junctional plaques as well as in internal vesicles. Images shown are single confocal slices. Scale bar applies to all images. (B) Tracer coupling measurements in HeLa cells transiently transfected with Cx36-Halo, Cx36-WT and HaloTag empty vector (EV). Ctrl, control; Sp, 5 mM Sp-8-cpt-cAMPS (PKA activator); Rp, 5 mM Rp-8-cpt-cAMPS (PKA inhibitor). Data are means±s.e.m., n=3 experiments per condition; *P<0.05, ***P<0.001.