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Pulse-chase labeling of HeLa cells transiently transfected with Cx36-Halo. Cells were labeled with OG (pulse; green label) at time 0 and TMR (chase; red label) at hours 0.3, 1, 2, 3, 5 and 6. Images are confocal stacks of 2.4–2.5 mm thickness. Scale bar applies to all images.
