Fig. 4.

Half-life of Cx36 in HeLa cells. (A) Calculation of Cx36 half-life in HeLa cells. The x-axis represents time lapsed; the y-axis is the natural log of total green intensity divided by total intensity of green plus red in gap junctions (intracellular vesicles are excluded); n=10–20 gap junctions per time point. Half-life is calculated by the natural log of 2 divided by the gradient of the best-fitted straight line. (B) Half-lives of Cx36 gap junction plaques were not altered by the treatment with 5 mM Sp-8-cpt-cAMPS or Rp-8-cpt-cAMPS throughout the chase period; n=3 experiments with 10–20 gap junctions per time point in each. Data are means±s.e.m.