Fig. 6.

Intracellular vesicles containing Cx36-Halo are readily tracked in the pulse-chase paradigm. Pulse (OG; green) and chase (TMR; red) ligands are the same as in Fig. 3. (A) The removal of Cx36 from the gap junction. Large removal vesicles were usually a mixture of OG- and TMR-labeled, and were often circular (arrows). The vesicles can be removed from the edge (ii: open arrowhead) as well as center (i: asterisk) of a pre-existing gap junction. (B) Small insertion vesicles (arrowheads) containing new Cx36 were often found docked on gap junctions, and were often adjacent to bright patches of chase labeling within the gap junction. The insertion can happen at the edge (i) or the center (ii) of an existing gap junction. Images Aii and Aiii are 2.5 mm thick stacks; all other images are single confocal slices. Scale bars are 2 µm in each panel.