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. 2015 Nov 1;128(21):3990–3996. doi: 10.1242/jcs.172882

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© 2015. Published by The Company of Biologists Ltd

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Fig. 4. — ER stress does not suppress the tgrC1⁻ and the tgrC1^QS38 phenotypes. We developed cells on dark nitrocellulose filters in the presence of one of the ER stress inducers, thapsigargin (Tg) or 17-N-Allylamino-17-demethoxygeldanamycin (17-AAG). Sporulation efficiency is the percentage of the total number of cells that formed spores. Bars show mean and s.e.m. (A) Treatment of wild-type (AX4) cells with the indicated concentrations of Tg or 17-AAG for 30 h (n=3). (B) 17-AAG treatment of AX4, tgrC1⁻ and tgrC1^QS38 cells for 72 h (n=3). (C) Thapsigargin treatment of AX4, tgrC1⁻ and tgrC1^QS38 cells for 72 h (n=3). Colored arrows (blue, tgrC1⁻; green, tgrC1^QS38) indicate bars with values below the limit of detection. Comparisons between bars (one-way ANOVA followed by Tukey's post hoc-test): *P≤0.05; **P≤0.01.