Fig. 5.

Allorecognition is not modified by the alg9^ins mutation nor by tunicamycin treatment. We developed the GFP- and RFP-labeled strains in chimera on non-nutrient agar. We took GFP and RFP images at 14–17 h of development and merged the images such that yellow indicates mixing of GFP- and RFP-labeled cells whereas green or red indicates segregated clonal populations. The relevant genotypes of the mixed strains are indicated. (A) GFP-labeled AX4 cells were developed in chimera with RFP-labeled strains as indicated above each panel. Scale bar=0.5 mm. (B) tgrB1^QS38/C1^QS38 GFP cells and tgrB1^AX4/C1^AX4 RFP cells were developed in chimera with the addition of tunicamycin at the indicated concentrations. Scale bar=0.5 mm.