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. 2015 Nov 1;128(21):3997–4013. doi: 10.1242/jcs.175539

Fig. 2.

Fig. 2.

Integrin αv does not localize to focal adhesions in keratinocytes. (A,B) Representative images of human fibroblasts (A) or human keratinocytes (B) stained for integrin ɑv, integrin β1 and paxillin, and/or incubated with phalloidin, then imaged with confocal microscopy. (C) Box plots showing quantification of the number of focal adhesions per cell for keratinocytes infected with a scramble hairpin, an integrin αv hairpin (shαv) or an integrin β1 hairpin (shβ1), and seeded onto coverslips coated with collagen, fibronectin or vitronectin (n=30–40 cells per condition, box-plot whisker ends are at the 1.5 interquartile range, boxes are at the 1st and 3rd quartile, and the line is at the median). P=2.45×10−12 (collagen), 8.15×10−15 (fibronectin), 2×10−16 (vitronectin) using one-way ANOVA. (D) Box plots showing quantification of cell spreading area for the same cells shown in C. P=3.79×10−9 (collagen), 2×10−16 (fibronectin), 2×10−16 (vitronectin) using one-way ANOVA. Scale bars: 100 μm. ***P<0.0005; NS, not significant.