Fig. 6.

RhoC controls phosphorylation of PLC-γ1 and eNOS and induces intracellular Ca⁺² release. HUVECs were transfected with control or RhoC siRNA, serum starved overnight, and treated with 10 ng/ml VEGF for 5, 10 or 15 min (+V5, +V10 and +V15, respectively). (A) Cell lysates were immunoblotted with antibodies against phosphorylated PLC-γ1 (pPLC-γ1) (Y783) and total PLC-γ-1. The corresponding densitometry graph is shown to the right. (B) Cell lysates were immunoblotted for phosphorylated eNOS (peNOS) (S1177), total eNOS and β-actin (loading control). The corresponding densitometry graph is shown to the right. (C) HUVECs transfected with control or RhoC siRNA were serum-starved overnight, loaded with Fura-2 AM and then stimulated with VEGF-A (10 ng/ml) at 50 s. All experiments were repeated at least three times.