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. 2015 Nov 17;5:16411. doi: 10.1038/srep16411

Figure 3. Biotransformation to SP by strain S16dspm, preparation of biocatalyst, and optimal conditions for biotransformation.

Figure 3

Preparation of biocatalysts using glycerin (■) and LB (●) medium, respectively. (A) Nicotine degradation in the initial stage of resting-cell reactions with glycerin and LB medium. (B) SP formation in the initial stage of resting-cell reactions with glycerin and LB medium. Effects of reaction conditions for SP production. (C) SP formation by strain S16dspm under whole-cell condition (●) and cell-free condition (■). (D) SP formation by strain S16dspm at various temperatures, 24 °C (■), 30 °C (●), 37 °C (▲), 42 °C (▼). (E) SP formation by strain S16dspm at various pH values, pH6 (■), pH7 (●), pH8 (▲), pH9 (▼). (F) SP formation by strain S16dspm with different initial nicotine contents, 15 g/L (■), 5 g/L (▲), 3 g/L (▼). Analysis of the biotransformation results at the optimal conditions using resting cells of S16dapm. (G) TLC and HPLC qualitative analysis of nicotine degradation and SP formation. (H) The content changes of nicotine and SP during the biotransformation process detected by HPLC quantitative analysis. Nicotine (■); SP (●).