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. 2015 Nov 17;5:16411. doi: 10.1038/srep16411

Figure 5. Time course of batch and fed-batch biotransformations by P. putida S16dspm.

Figure 5

(A) For batch biotransformation of aqueous nicotine solution, the biocatalysts were reused three times. Nicotine (black) and SP (red) concentration were analyzed by HPLC at the end of each batch reaction. (B) Time course of fed-batch biotransformation from aqueous nicotine solution by P. putida S16dspm at pH 9.0, 24 °C, and 5.6 mg/ml DCW resting cells. Nicotine (■); SP (●). (C–E) Time course of fed-batch biotransformation by P. putida S16dspm from crude tobacco extract at pH 5.1 (C), pH 7.0 (D), and pH 9.0 (E). The fed-batch biotransformation from crude tobacco extract were performed at different pH conditions. Nicotine (■); SP (●).