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. 2015 Nov 1;142(21):3746–3757. doi: 10.1242/dev.119909

Fig. 6.

Fig. 6.

EMX1 regulates SEMA3C-mediated chemoattraction of callosal axons from the cingulate cortex. (A-D) Cingulate cortical explants were dissected from E15.5 Emx1+/+/C57Bl/6 and Emx1−/−/C57Bl/6 mice to examine callosal axon guidance in vitro. Explants were co-cultured with either control HEK293T-cell blocks or SEMA3C-expressing HEK293T-cell blocks (white arrows point towards the cell blocks). (E) Explants from Emx1+/+/C57Bl/6 animals were significantly attracted by SEMA3C-expressing cell blocks compared with control cell blocks. By contrast, SEMA3C did not significantly attract axons from Emx1−/−/C57Bl/6 cingulate cortex. (F) Outgrowth of cingulate cortical axons was unaffected by SEMA3C. All SEMA3C guidance values were normalized to control values in E. Total explant n values pooled from three independent experiments are displayed within the columns in F. *P<0.05 for two-tailed Student's t-test. Results are mean±s.e.m. Scale bar: in D, 200 μm for A-D.