Skip to main content
. 2015 Nov 1;142(21):3734–3745. doi: 10.1242/dev.125328

Fig. 4.

Fig. 4.

Cleft palate is a consequence of tongue and mandible malformations in Wnt1-Cre;Erk2fl/fl mice. (A) Quantitative analysis of Erk2 expression in the palatal shelves and mandible of control and Osr2-Cre;Erk2fl/fl mice at E13.5. *P<0.05. (B) Macroscopic views of control and Osr2-Cre;Erk2fl/fl newborns. Arrow indicates milk in the stomach of the Osr2-Cre;Erk2fl/fl mouse. (C-F) Intraoral views of the tongues, mandibles and palates of control and Osr2-Cre;Erk2fl/fl newborns. (G,H) HE staining of coronal sections of E16.5 control and Osr2-Cre;Erk2fl/fl mice. (I,J) Intraoral views of heads from E13.5 control and Wnt1-Cre;Erk2fl/fl embryos from which the mandible and tongue were removed and cultured for 3 days (n=4). Dashed lines delineate the palatal shelves. (K,L) K14 immunostaining (red) of coronal sections of E13.5 cultured explants. (M,N). Intraoral views of heads from E14.0 control and Wnt1-Cre;Erk2fl/fl embryos from which the mandible and tongue were removed and cultured for 3 days (n=3). (O,P) K14 immunostaining (red) of coronal sections of E14.0 cultured explants. Arrowhead in O points to the midline of the control sample, where palatal shelves are fused and no epithelial cells remain. Scale bars: 100 µm.