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. 2015 Nov 1;142(21):3734–3745. doi: 10.1242/dev.125328

Fig. 5.

Fig. 5.

Proliferation and differentiation are unaffected in Wnt1-Cre;Erk2fl/fl tongues. (A-F) PH3 (red) and Myod1 (green) double immunostaining of coronal sections of E12.5-E14.5 control and Wnt1-Cre;Erk2fl/fl tongues. (G) Quantification of proliferation (PH3+) in Myod1-positive myogenic cells (Myo) and Myod1-negative cranial neural crest cells (CNCC) from A-F. Dark bars, control tongues; light bars, Wnt1-Cre;Erk2fl/fl tongues. (H-M) MHC immunostaining (green) of coronal sections of E12.5-E14.5 control and Wnt1-Cre;Erk2fl/fl tongues. Arrows point to the extrinsic muscles and arrowheads point to the intrinsic muscles. (N,O) Quantitation of real-time qPCR of Myog and Mrf4 in control (gray bars) and Wnt1-Cre;Erk2fl/fl (blue bars) tongues at E13.5 and E14.5. n=3 for each stage and experiment. Scale bars: 100 µm.