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. 2015 Nov 1;142(21):3734–3745. doi: 10.1242/dev.125328

Fig. 6.

Fig. 6.

Cranial neural crest cell-derived tendons are abnormal in Wnt1-Cre;Erk2fl/fl tongues. (A-D) In situ hybridization of scleraxis (Scx) in coronal sections of E13.5 and E15.5 control and Wnt1-Cre;Erk2fl/fl heads. Arrows indicate Scx expression. (E-H) Double immunostaining of Sp7 (red) and MHC (green) in coronal sections of E13.5 and E15.5 control and Wnt1-Cre;Erk2fl/fl heads. Dashed lines delineate the Meckel's cartilage. The arrowhead in H indicates the abnormally close proximity of the extrinsic muscles of the tongue to the Meckel's cartilage and osteogenic progenitors. n=3 for each stage and analysis. Scale bars: 100 µm.