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. 2015 Nov 1;142(21):3734–3745. doi: 10.1242/dev.125328

Fig. 8.

Fig. 8.

The onset of mandibular defects precedes palatal shelf elevation. (A-D) Sagittal views of 3D reconstructions from microCT scans (A,B) and Alizarin Red/Alcian Blue staining (C,D) of newborn control and Wnt1-Cre;Erk2fl/fl skulls. Arrow in D points to the malformed mandible in Erk2 mutant mice. (E-H) Intraoral views of 3D reconstructions from microCT scans (E,F) and Alizarin Red/Alcian Blue staining (G,H) of newborn control and Wnt1-Cre;Erk2fl/fl mandibles. (I) Quantification of the volume of control and Wnt1-Cre;Erk2fl/fl mandibles from 3D reconstructions. *P<0.05. (J-M) Macroscopic sagittal views of E13.5 and E14.5 control and Wnt1-Cre;Erk2fl/fl embryos. Arrowheads indicate malformed mandibles. (N-Q) Skeletal staining of skulls and mandibles from E14.5 control and Wnt1-Cre;Erk2fl/fl embryos. n=3 for each stage. Scale bars: 1 mm.